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It is becoming widely recognized, however, that some common oilfield SRB strains do not grow on lactate. In addition, some SRB strains exhibit elaborate trace organic requirements that must be met before they will grow. As a result, an increasing variety of media and incubation conditions are being used to detect and cultivate SRB.

As media formulations become more complex, however, particularly as to potential sulfur sources, the risk of obtaining false results increases. Therefore, selecting a medium solely because it recovers higher populations of sulfide-producing bacteria does not mean that SRB are also recovered efficiently.

As media formulas become more complex, biological communities or "consortia" can develop in the media. These consortia can produce sulfide from sulfite, thiosulfate, cysteine hydrochloride, and other sulfur-containing compounds that might be added to the medium. In fact, SRB may not be present even when a positive result is obtained in such media. The key point is, unless the sulfide is generated stoichiometry from sulfate under anaerobic conditions , then bacteria other than SRB are involved.

Therefore, a simple defined medium with sulfate as the only sulfur source is preferable to grow SRB. However, this leads to a problem: while simple defined media are preferable for proving that SRB are present, many SRB's will not grow on these media.

Clearly, rapid, accurate detection of SRB is highly desirable. It was discovered recently that all eubacterial SRB share a common enzyme, adenosine 5-phosphosulfate reductase APS reductase , which catalyzes the reduction of adenosine 5-phosphosulfate to sulfite and adenosine monophosphate. APS reductase is required for respiratory sulfate reduction i. APS reductase is not used in assimilatory sulfate reduction and thus is not present in non-SRB eubacteria.

This enzyme is present in some colorless sulfur bacteria and photosynthetic present in some colorless sulfur bacteria and photosynthetic microorganisms but not in Significant quantities. It is not known whether the enzyme would be present in any archaebacterial SRB that might be discovered in the future. With APS reductase as a marker, a highly selective and sensitive immunoassay method was developed and patented to detect SRB.

Non-SRB bacteria, including sulfur and sulfite-oxidizing bacteria, do not cross-react with the immunoassay or otherwise impede accurate SRB determinations. An SRB field test kit was developed from this immunoassay. Tatnall et al. Horacek and Gawel also reported field tests of an early version of the field test kit. That testing showed that the presence of other bacteria, oil, chemicals, oxidized metals, and sulfide did not interfere with the performance of the test kit in oilfield waters.

This paper presents field data obtained with the now commercially available version of the rapid test method. The SRB rapid was field evaluated within several geographically diverse company operating divisions. Water samples generally were collected from producing wells, battery tanks, and various points within water-injection producing wells, battery tanks, and various points within water-injection systems.

Usually these samples contained nominal quantities of oil. Depending on their source, some samples also contain small amounts of such normal production chemicals as emulsion breakers, reverse breakers, and corrosion inhibitors. All fluids were tested in an unaltered state. Specifications and information herein are subject to change without notice.

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